Serotyping of Dengue Virus from Deadly Outbreaks of Pakistan
ثبت نشده
چکیده
Dengue Dengue virus (DENV) belonging to the family Flaviviridae consists of 4 serotypes (DEN-1, 2, 3 & 4) causing severe illnesses like dengue fever (DF), Dengue Hemorrhagic Fever (DHF) and Dengue Shock Syndrome (DSS). Dengue fever has become an emerging disease in Pakistan during the past 5-7 years with an increase in the number of cases every year since 2006. The current study aimed at identifying the serotypes of dengue virus involved in 2011 outbreak of Pakistan. Samples were collected from all provinces of Pakistan. Patients were confirmed in the hospital by antibody captured Enzyme linked immunosorbent assay (ELISA). These ELISA positive serum samples were inoculated on HeLa cell line to raise the viral titer of dengue virus and then RT-PCR was done for serotyping of dengue virus. Initially D1, D2 primers were tried for serotyping of dengue virus but poor results were obtained.
منابع مشابه
Characterization of dengue virus in Aedes aegypti and Aedes albopictus spp. of mosquitoes: A study in Khyber Pakhtunkhwa, Pakistan
Dengue is a vector-borne disease caused by dengue virus. According to the recent report of CDC that one-third population of the world are at high risk with Dengue fever. The prevalence of the dengue hemorrhagic fever was found more in tropical and sub-tropical regions of the world. Aedes mosquitoes was reported as the main cause of transmission of dengue virus. So the current study was planned ...
متن کاملIsolation and Serotyping of Dengue Viruses by Mosquito Inoculation and Cell Culture Technique: An Experience in Bangladesh
Dengue virus isolation and serotyping was carried out in 105 clinically-suspected cases of dengue fever by the mosquito inoculation and cell culture techniques to find a suitable virus isolation technique for a resource-constrained country like Bangladesh and to identify the serotypes responsible for the dengue outbreak in 2000. Virus was identified by direct fluorescent antibody technique (DFA...
متن کاملCOVID-19 Outbreak in Africa: Lessons and Insights from the West African Ebola Virus Disease Epidemics
Over the years, the African continent has had to battle several outbreaks of infectious diseases in different countries. Some of the most deadly were the Ebola virus disease (EVD) outbreaks that occurred in West Africa between 2014 and 2016 affecting Guinea, Liberia, and Sierra Leone and, more recently, from 2018 to 2020 in the Democratic Republic of Congo (DRC). In the era of the COVID-19 pand...
متن کاملDengue Fever Serology in Febrile Patients in Southeast Iran
Background: Dengue is a mosquito-borne viral disease that has rapidly spread in all regions in recent years. There is little information on dengue fever epidemiology in Iran. High prevalence of dengue fever in Pakistan bordering southeast Iran emphasizes the need for paying more attention to monitoring of the disease in this region. The aim of this study was to study the dengue...
متن کاملDengue Virus Serotypes Circulating in Khyber Pakhtunkhwa Province, Pakistan, 2013-2015
From 2013 to 2015, the National Institute of Health, Pakistan, received 1,270 blood samples of suspected dengue cases reported from inpatient and outpatient departments of various hospitals in Khyber Pakhtunkhwa (KPK) province. In this study, we determined the circulating dengue virus (DENV) serotypes using real-time reverse transcriptase (RT)-PCR to understand the serotype-based epidemiology o...
متن کامل